Rapid Identification of a Protein Binding Partner for the Marine Natural Product Kahalalide F by Using Reverse Chemical Proteomics (p 524-530)
Andrew M. Piggott, Peter Karuso
Published Online: Jan 25 2008 7:14AM
DOI: 10.1002/cbic.200700608
Capable chemists: Chemists are playing an ever more important role in biology through the application of small molecules to biological systems. In this paper, we combine affinity chromatography and phage display (reverse chemical proteomics), to isolate a cellular receptor for the new anticancer drug and marine natural product, kahalalide F. The results challenge biologists to uncover the significance of the interaction between the drug and its target protein.